Journal: Cancer Immunology, Immunotherapy : CII

Article Title: A novel murine model of allogeneic vaccination against prostate cancer

doi: 10.1007/s00262-007-0384-2

Figure Lengend Snippet: Characterisation of prostate cell lines PMC-1 and PMC-1 C6ras1p1

Article Snippet: They were blocked with 10% normal serum (Dako, Ely, UK) corresponding to the species of secondary antibody for 15 min; 100 μl of pre-titrated primary antibody [Cytokeratin 18 (RDI, Flanders, USA), Desmin (RDI), Vimentin (Santa Cruz, Wembley, UK), Androgen receptor (Santa Cruz), Anti-cytokeratin cocktail (ICC staining kit) (Nexell Therapeutics Inc.), MOPC21 isotype control (Dako), normal mouse IgG isotype control (ICC staining kit) (Nexell Therapeutics Inc.) was then added and incubated for 45 min at room temperature; 100 μl of pre-titrated secondary antibody [Goat anti-mouse (Jackson Immunology Research), Rabbit anti-goat (Jackson), Goat anti-rabbit (Jackson), Goat anti-mouse (ICC staining kit) (Nexell)] conjugated with alkaline phosphatase was added and incubated for 30 min. After two washes with TBS (Tris buffer solution), component A (Naphtol AS phosphate and Levamisole in Tris buffer, pH8.5) and B (Fast Red Violet salt) from the ICC staining kit were mixed, applied and incubated for 20 min at room temperature.

Techniques:

Journal: Cells

Article Title: The Functional Role and Regulatory Mechanism of Bromodomain-Containing Protein 9 in Human Uterine Leiomyosarcoma

doi: 10.3390/cells11142160

Figure Lengend Snippet: Morphological observation and IHC staining of SMA, desmin, and HMB45 in four representative human uLMS tissues and adjacent myometrium.

Article Snippet: Desmin , Santa Cruz , SC-14026 , Rabbit , IHC-P , 1:800.

Techniques: Immunohistochemistry